Gelatin, Skin

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular weights, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue and Type B gelatin is derived from lime-cured tissue. The charge on a gelatin molecule and its isoelectric point are primarily due to the carboxyl amino and guanidino groups on the side chains. Type A gelatin has 78-80 millimoles of free carboxyl groups per 100g of protein and a pI of 7.0-9.0; type B has 100-115 millimoles of free carboxyl groups per 100g of protein and a pI of 4.7-5.2. The pH of a 1.5% solution at 25 degrees C is 3.8-5.5 for Type A and 5.0-7.5 for Type B. It is soluble in glycerol and acetic acid, and more soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. \n\nApplications:\nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:1000-1:10,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G2024-01A

Size

100ul

Applications

ELISA

Hosts

Rabbit

Reactivities

Prc

Form

Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Merck Index, 12th Ed., S. Budavari, Ed., p. 742, #4388 (1996). 2. Standard Methods for the Sampling and Testing of Gelatins, Gelatin Manufacturers Institute of America, Inc., 501 fifth Ave., Room 1015, New York, NY. Vogt, R. F. (1987) J. Immunol. Methods 101, 43. 3. Levine, M. (1923) J. Bacteriol. 8, 297. 4. Sakai, Y (1998) Biol. Pharm. Bull. 21, 330. 5. Young, S. (2005) Control Release 109, 256-274 (2005). 6. Huang Y (2005) Biomaterials 26, 7616-7627 (2005).

Additional Info

Recognizes porcine skin gelatin