Gelsolin (Actin depolymerizing factor, ADF, AGEL, Amyloidosis Finnish type, Brevin, DKFZp313L0718, GSN, Calcium regulated actin binding protein)

Gelsolin participates in the extracellular actin scavenger system, binding actin and disaggregating filamentous actin released from dying cells. Actin monomers are then transferred to Gc-globulin. The plasma concentration of gelsolin is reduced in conditions of major actin release, such as acute liver failure, major trauma and rhabdomyolysis, where multi-organ failure is a risk. It has been suggested that gelsolin, like Gc-globulin, can be a prognostic factor in these conditions.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:2000 applies to gelsolin presented on a coat of polyclonal capture antibody. \nNote: Does not work as capture antibody in sandwich ELISA.\nOptimal dilutions to be determined by the researcher.\n\nCulture medium: \nRPMI 1640 with 10% calf serum\n\nHybridoma: \nSP2mlL6. Female CF1 x Balb/c mice immunized i.p. with immunogen adsorbed onto Al(OH)3 and emulsified in Freund

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SPECIFICATIONS

Catalog Number

G2024-52

Size

100ul

Applications

ELISA

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%.\n

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG1,k

References

1. Lee PS, Drager LR, Stossel TP, Moore FD, Rogers SO (2006) Relationship of plasma gelsolin levels to outcomes in critically ill surgical patients. Ann Surg 243:399-403.

Additional Info

Recognizes human gelsolin.