Gelsolin (AGEL, ADF, Brevin, Actin-depolymerizing factor)

Gelsolin, a protein of leukocytes, platelets, and other cells, severs actin filaments in the presence of submicromolar calcium, thereby solating cytoplasmic actin gels. A gelsolin variant with 23 more N-terminal amino acids is a plasma component probably involved in the clearance of actin, the most abundant human protein, from the circulation. Gelsolin is located in 9q34. Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain.\n\nUniProt Number:\nHuman(P06396), Mouse(P13020), Rat(Q68FP1)\n\nGene ID:\nGSN\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry (Paraffin and Frozen).\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144568

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Rabbit

Form

Ascites, supplied as a lyophilized powder. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.

P Type

Mab

Isotype

IgG1

References

1. Kwiatkowski, D. J.; Ozelius, L.; Schuback, D.; Gusella, J.; Breakefield, X. O. : The gelsolin (GSN) cDNA clone, from 9q32-34, identifies BclI and StuI RFLPs. Nucleic Acids Res. 17: 4425 only, 1989.\n2. Kwiatkowski, D. J.; Westbrook, C. A.; Bruns, G. A. P.; Morton, C. C. : Localization of gelsolin proximal to ABL on chromosome 9. Am. J. Hum. Genet. 42: 565-572, 1988.\n3. Kwiatkowski, D. J.; Stossel, T. P.; Orkin, S. H.; Mole, J. E.; Colten, H. R.; Yin, H. L. : Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain. Nature 323: 455-458, 1986.

Additional Info

Recognizes human, and rabbit Gelsolin. No cross reactivity with other proteins.