Geminin (GMNN, GEM, Geminin DNA Replication Inhibitor, DNA replication inhibitor, RP3 369A17.3)

Geminin inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex. It is degraded during the mitotic phase of the cell cycle, has a destruction box sequence (RRTLKVIQP), and is ubiquitinated by the anaphase promoting complex in vitro. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Further, geminin interacts tightly with CDT1, a replication initiation factor necessary for MCM loading.\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nImmunoprecipitation: 2-5ug/mg lysate\nWestern Blot: 1:2000-1:10,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

G2024-55E

Size

100ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in Tris-citrate/phosphate buffer, pH 7.5, 0.09% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Additional Info

Recognizes human Geminin. The epitope maps to a region between residue 160 and the C-terminus (residue 209) of human Geminin DNA replication Inhibitor using the numbering given in entry NP_056979.1 (GeneID 51053).

Alternative Names

GMNN, GEM, Geminin DNA Replication Inhibitor, DNA replication inhibitor, OTTHUMP00000039393, RP3 369A17.3