Germ Cell Nuclear Factor

Germ cell nuclear factor (GCNF), a NR6 Germ Cell Nuclear Factor-Like Receptor, has been shown to affect gene expression in embryogenesis and spermatogenesis and is essential for embryonic survival and normal development. Loss of GCNF causes embryonic lethality and disrupts normal somitogenesis as well as neural-tube and axis formation. GCNF binds as a homodimer to the DR0 DNA response element and has been shown to regulate transcription of genes including protamines1 and 2 and Oct4. GCNF's repression function has been shown to be mediated by interaction with the co-repressors N-COR and SMRT in the absence of ligand. Three protein isoforms, GCNF-1, GCNF-2a, and GCNF-2b, have been documented in humans, and they are generated by differential usage of alternative splice acceptor sites of the fourth and seventh exons. GCNF-1, the longest isoform, has a distinct 5 UTR and contains 4 and 26 additional amino acids than the other two forms, respectively. GCNF expression has been documented by in human testis, ovary, prostate, small intesine, and colon. Little or no expression was identified in brain, heart, liver, lung, pancreas, placenta, skeletal muscle, spleen, or thymus. ESTs have been isolated from human tissue libraries, including cancerous cervix, colon, placenta, and nerve in addition to normal testis and embryo. Applications: Suitable for use in Immunohistochemistry (Fr) (paraffin-embedded sections). Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.