Home  >  Products  >  Germ Cell Nuclear Factor (GCNF, GCNF1, hGCNF, Nuclear Receptor Subfamily 6 Group A Member 1, NR6A1, NR61, Retinoid Receptor-related Testis-specific Receptor, RTR, hRTR)
Germ Cell Nuclear Factor (GCNF, GCNF1, hGCNF, Nuclear Receptor Subfamily 6 Group A Member 1, NR6A1, NR61, Retinoid Receptor-related Testis-specific Receptor, RTR, hRTR)

Germ Cell Nuclear Factor (GCNF, GCNF1, hGCNF, Nuclear Receptor Subfamily 6 Group A Member 1, NR6A1, NR61, Retinoid Receptor-related Testis-specific Receptor, RTR, hRTR)

Cat no: G3010-01

Supplier: United States Biological
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GCNF (Germ Cell Nuclear Factor), also known as NR6A1 (Nuclear Receptor Subfamily 6 Group A member), is an orphan member of the nuclear receptor gene superfamily (1). It has been shown to be expressed in the nervous system during development and during specific stages in maturing germ cells of the ovary and testis in the adult, and has probable roles in ametogenesis, neurogenesis, and normal embryonic development during gastrulation (1,2). Inactivation of GCNF in mouse results in abnormal posterior development, impaired midbrain development, insufficient closure of the neural tube, and eventual embryonic death (3). GCNF has been shown to be a repressor of OCT-4 and of the protamine genes (4,5) and plays a critical role in the control of gene expression during embryogenesis and spermatogenesis (2,6). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: G3010-01
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A affinity chromatography.
References: (1) Lan, Z.J. et al. (2009) Biol Reprod 80, 905-12. (2) Lei, W. et al. (1997) J Mol Endocrinol 18, 167-76. (3) Chung, A.C. et al. (2001) Mol Cell Biol 21, 663-77. (4) Akamatsu, W. et al. (2009) J Neurosci 29, 2113-24. (5) Hummelke, G.C. and Cooney, A.J. (2004) Mol Reprod Dev 68, 394-407. (6) Lan, Z.J. et al. (2002) J Biol Chem 277, 50660-7.
Additional info: Recognizes endogenous levels of total GCNF. Species Crossreactivity: mouse, rat.

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