Germ Cell Nuclear Factor (GCNF, GCNF1, hGCNF, Nuclear Receptor Subfamily 6 Group A Member 1, NR6A1, NR61, Retinoid Receptor-related Testis-specific Receptor, RTR, hRTR)

GCNF (Germ Cell Nuclear Factor), also known as NR6A1 (Nuclear Receptor Subfamily 6 Group\nA member), is an orphan member of the nuclear receptor gene superfamily (1). It has been shown to be expressed in the nervous system during development and during specific stages in maturing germ cells of the ovary and testis in the adult, and has probable roles in ametogenesis,\nneurogenesis, and normal embryonic development during gastrulation (1,2). Inactivation of GCNF in mouse results in abnormal posterior development, impaired midbrain development, insufficient closure of the neural tube, and eventual embryonic death (3). GCNF has been shown to be a repressor of OCT-4 and of the protamine genes (4,5) and plays a critical role in the control of gene expression during embryogenesis and spermatogenesis (2,6).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

G3010-01

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

(1) Lan, Z.J. et al. (2009) Biol Reprod 80, 905-12. (2) Lei, W. et al. (1997) J Mol Endocrinol 18, 167-76.\n(3) Chung, A.C. et al. (2001) Mol Cell Biol 21, 663-77. (4) Akamatsu, W. et al. (2009) J Neurosci 29, 2113-24. (5) Hummelke, G.C. and Cooney, A.J. (2004) Mol Reprod Dev 68, 394-407. (6) Lan, Z.J. et al. (2002) J Biol Chem 277, 50660-7.

Additional Info

Recognizes endogenous levels of total GCNF. Species Crossreactivity: mouse, rat.