Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry (paraffin), and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:50000-100000
Immunohistochemistry (paraffin): 1:250-500
Immunocytochemistry: 1:100-250
Immunoprecipitation: 1:40
Optimal dilutions to be determined by the researcher.
Positive Control:
Human and rat brain lysate
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.