Green fluorescent protein (GFP) is a 27kD protein, which was originally cloned from Jellyfish cnidarians, Aequorea victoria. This exceptional protein absorbs blue light (maximally at 395nm) and emits green light (peak at 509nm) without any requirement of exogenous substrates and cofactors. These unique qualities of its intrinsic fluorescence make GFP become an invaluable tool in cell biology research such as monitoring gene expression and protein localization of GFP-tagged proteins in vivo. Other applications of GFP also include assessment of protein-protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. Several mutant forms of GFP have been developed which fluoresce more intensely and have shifted excitation maximum when compared to the wild type GFP, making them useful for FACS, fluorescence microscopy, and double-labeling applications.
Applications:
Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
ELISA: 1:5000-1:20,000
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:500-1:1000 (1-2ug/ml)
Immunoprecipitation: 2-5ug/10e7 cells extract
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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