Home  >  Products  >  GFPT1 (Glucosamine-fructose-6-phosphate Aminotransferase [Isomerizing] 1, GFPT, Glutamine:fructose 6 Phosphate Amidotransferase 1, GFAT 1, GFAT1, GFAT1m, GFA, GFAT, D-fructose-6-phosphate Amidotransferase 1, Hexosephosphate Aminotransferase 1)
GFPT1 (Glucosamine-fructose-6-phosphate Aminotransferase [Isomerizing] 1, GFPT, Glutamine:fructose 6 Phosphate Amidotransferase 1, GFAT 1, GFAT1, GFAT1m, GFA, GFAT, D-fructose-6-phosphate Amidotransferase 1, Hexosephosphate Aminotransferase 1)

GFPT1 (Glucosamine-fructose-6-phosphate Aminotransferase [Isomerizing] 1, GFPT, Glutamine:fructose 6 Phosphate Amidotransferase 1, GFAT 1, GFAT1, GFAT1m, GFA, GFAT, D-fructose-6-phosphate Amidotransferase 1, Hexosephosphate Aminotransferase 1)

Cat no: G2032-90H

Supplier: United States Biological
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GFAT1, glutamine:fructose-6-phosphate aminotransferase 1, is the rate-limiting enzyme of the hexosamine biosynthesis pathway (1). This enzyme catalyzes the conversion of fructose-6-phosphate and glutamine to glucosamine-6-phosphate and glutamate (2). The hexosamine biosynthesis pathway generates the building blocks for protein and lipid glycosylation (2). Furthermore, studies suggest that increased activity of this pathway is a contributing factor to hyperglycemia-induced insulin resistance (1,2). GFAT1 is more active in non-insulin-dependent diabetes mellitus (NIDDM) patients (3). Transgenice mice overexpressing this enzyme in skeletal muscle and adipose tissue show an insulin resistance phenotype (4,5). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: G2032-90H
Reactivities: Human, Rat
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.
P type: Mab
Isotype: IgG
Purity: Purified
Alternative names: EC=2.6.1.16
References: (1) Niimi, M. et al. (2001) J Hum Genet 46, 566-71. (2) DeHaven, J.E. et al. (2001) Diabetes 50,2419-24. (3) Yki-J
Additional info: Recognizes endogenous levels of total GFAT1. Species Crossreactivity: rat.

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