GFR alpha1 (Glial Cell Line Derived Neurotrophic Factor Receptor Alpha 1, GDNF Family Receptor alpha 1, GDNF Receptor alpha 1, GFR-alpha-1, GFRalpha1, GFRA1, GDNF Receptor alpha, GDNFR alpha, GDNFR-alpha, GDNFRa, GDNFR, GPI-linked Anchor Protein, MGC23045

Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons and is essential for the development of kidneys and the enteric nerves system. Physiological responses to GDNF require the presence of a novel glycosylphosphadidylinositol linked protein GDNFRalpha, which is a cell surface receptor for GDNF (1,2). The cDNAs encoding GDNFRalpha from human, rat, chicken and mouse have been cloned recently (1-5). GDNFRalpha was also termed Ret ligand 1 (RETL1) or TGF-beta-related neurotrophic factor receptor 1 (TrnR1) and nominated as GFRalpha-1 recently (5-7). GFRalpha-1 binds GDNF specifically and mediates activation of the Ret protein tyrosine kinase (PTK). Thus, GDNF, GFRalpha and the Ret PTK form a complex to transduce GDNF signal and to mediate GDNF function.\n \nApplications:\nSuitable for use in Western Blot. Other applications not tested. \n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G2033-02B

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

P Type

Pab

Purity

Purified immunoglobulin

Isotype

IgG

References

1. Jing S; Wen D; Yu Y; Holst PL ; Luo Y; Fang M; Tamir R; Antonio L; Hu Z; Cupples R; Louis JC; Hu S; Altrock BW; Fox GM. GDNF-induced activation of the Ret protein tyrosien kinase is mediated by GDNFR-?, a novel receptor for GDNF. Cell, 1996;85:1113-24. 2. Treanor JJS; GoodmanL; Sauvage FD; Stone DM; Poulsen KT; Beck CD; Gray C; Armanini MP; Pollock RA; Herti F; Phillips HS; Goddard A; Moore MW; Buj-Bello A;Davies AM; Asai N; Takahashi M; Vandlen R; Henderson CE; Rosenthal A. Characterization of a multicomponent receptor for GDNF. Nature, 1996;382:80-83. 3. Sanicola M, Hession C, Worley D, Carmillo P, Ehrenfels C, Walus L, Robinson S, Jaworski G, Wei H, Tizard R, Whitty A, Pepinsky RB, Cate RL. Glial cell line-derived neurotrophic factor-dependent RET activation can be mediated by two different cell-surface accessory proteins. Proc Natl Acad Sci U S. 1997;94:6238-6243 4. Buj-Bello A, Adu J, Pinon LG, Horton A, Thompson J, Rosenthal A, Chinchetru M, Buchman VL, Davies AM. Neurturin responsiveness requires a GPI-linked receptor and the Ret receptor tyrosine kinase. Nature 1997;387:721-724 5. Dey,B.K., Wong,Y.W. and Too,H.P. Cloning of a novel murine isoform of the glial cell line-derived neurotrophic factor receptor. Neuroreport 1998;9:37-42 6. Baloh RH, Tansey MG, Golden JP, Creedon DJ, Heuckeroth RO, Keck CL, ZimonjicDB, Popescu NC, Johnson EM Jr, Milbrandt J. TrnR2, a novel receptor that mediates neurturin and GDNF signaling through Ret. Neuron 1997;18:793-802

Additional Info

Recognizes human GFR alpha 1. Species Crossreactivity: Mouse and rat.

Alternative Names

Glial Cell Line-derived Neurotrophic Factor Receptor alpha