GGT1 (Gamma-glutamyltranspeptidase 1, GGT 1, GGT, CD224, D22S672, D22S732, Gamma-glutamyltransferase 1, GTG, MGC96892, MGC96904, MGC96963)

The enzyme encoded by this gene catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein, and is present in tissues involved in absorption and secretion. This enzyme is a member of the gamma-glutamyltransferase protein family, of which many members have not yet been fully characterized and some of which may represent pseudogenes. This gene is classified as type I gamma-glutamyltransferase. Multiple alternatively spliced variants, encoding the same protein, have been identified.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nAA Sequence:\nTAHLSVVAEDGSAVSATSTINLYFGSKVRSPVSGILFNNEMDDFSSPSITNEFGVPPSPANFIQPGKQPLSSMCPTIMVGQDGQVRMVVG\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

127288

Size

100ug

Applications

ELISA, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a,k

References

1. Autocatalytic cleavage of human {gamma}-glutamyl transpeptidase is highly dependent on N-glycosylation at asparagine 95. West MB, Wickham S, Quinalty LM, Pavlovicz RE, Li C, Hanigan MH.J Biol Chem. 2011 Jun 28. [Epub ahead of print] 2. Analysis of site-specific glycosylation of renal and hepatic ?^-glutamyl transpeptidase from normal human tissue. West MB, Segu ZM, Feasley CL, Kang P, Klouckova I, Li C, Novotny MV, West CM, Mechref Y, Hanigan MH.J Biol Chem. 2010 Sep 17;285(38):29511-24. Epub 2010 Jul 9.

Additional Info

Recognizes human GGT1. Species Crossreactivity: mouse.