GGTB2 (Beta-1,4-galactosyltransferase 1, Beta-1,4-GalTase 1, Beta4Gal-T1, b4Gal-T1, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1, Processed beta-1,4-galactosyltransferase 1,

Beta-1, 4-galactosyltransferase 1 (B4GalT1) is one of seven B4GalT genes. Human B4GalT1 encodes a type II membrane protein, which is ubiquitously expressed and involved in glycoconjugate biosynthesis. A soluble form (aa78-398), derived from proteolytic cleavage, is a component of lactose synthase in milk that also contains alpha-lactalbumin. The amino acid sequence of human B4GALT1 is 88%, 87% and 71% identical to that of rat, mouse/canine and chicken.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation and ELISA. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml The detection limit for recombinant human B4GaIT1 is ~5ng/lane under non-reducing and reducing conditions using a colormetric detection system. \nImmunoprecipitation: 25ug/ml immunoprecipitates recombinant human B4GaIT1 from conditioned cell culture medium. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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