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Ghrelin O-acyltransferase (GOAT, FKSG89, Membrane-bound O-acyltransferase Domain-containing Protein 4, MBOAT4, O-acyltransferase Domain-containing Protein 4, OACT4)

Cat no: G2033-83B


Supplier: United States Biological
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Ghrelin, an orexigenic hormone secreted from the stomach, exists in the circulation in two isoforms: des-acyl and acyl ghrelin. Acylation by the enzyme ghrelin O-acyl-transferase (GOAT) enables ghrelin to activate the ghrelin receptor. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Formalin-fixed paraffin): 1:500, 1hour at RT (DAB). Block using 3% H2O2, 2% normal goat serum. No pretreatment of section is required. Optimal dilutions to be determined by the researcher. Positive Control: Human stomach tissues Recommended Secondary Antibodies: I1904-40H: IgG, Fc (Biotin) Pab Gt x Rb I1904-41C: IgG, F(ab')2 (Biotin) Pab Gt x Rb Recommended Secondary Reagents: S7973-97B: Streptavidin (HRP) S7973-89H: Streptavidin (HRP) S7973-89V: Streptavidin (HRP) S7973-97D: Streptavidin (HRP) Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: G2033-83B
Reactivities: Human
Hosts: Rabbit
Applications: Immunohistochemistry
Size: 100ul
Form: Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O.
P type: Pab
Isotype: IgG
Purity: Serum
Alternative names: EC=2.3.1.-
References: 1. Current Opinion in Endocrinology, Diabetes & Obesity 18(1): 50
Additional info: Recognizes human Ghrelin O-acyltransferase.

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