Ghrelin, Rat, Mouse, BioAssay(TM) ELISA Kit

Supplier:

United States Biological

Cat no: G2033-73

Ghrelin, Rat, Mouse, BioAssay(TM) ELISA Kit

This Enzyme Immunoassay kit is designed to detect a specific peptide and its related peptides based on the principle of "competitive" enzyme immunoassay. Plasma, serum, culture media, tissue homogenate, CSF, urine or any biological fluid can be assayed as long as the level of the sample is high enough for the sensitivity of the kit to detect.\n\nPlasma Extraction: Extraction is strongly recommended but not required.\n\nPeptide Sequence used in kit:пїЅ\nGly-Ser-Ser(Octanoyl)-Phe-Leu-Ser-Pro-Glu-His-Gln-Lys-Ala-Gln-Gln-Arg-Lys-Glu-Ser-Lys-Lys-Pro-Pro-Ala-Lys-Leu-Gln-Pro-Arg\n\nKit Components:\nG2033-73A: 96-well Microplate1x1plate\nG2033-73B: Assay Buffer (20X) 1x50ml\nG2033-73C: Serum 1x5ml\nG2033-73D: Standard1x1ug\nG2033-73E: Peptide (Biotin) 1x5ml\nG2033-73F: Streptavidin (HRP) 1x30ul\nG2033-73G: Substrate solution 1x12ml\nG2033-73H: Stop Solution, 2N HCI 1x15ml\nG2033-73J: Positive Control 2x200ul\n\nSensitivity: \nMinimum Detectable Concentration: 0.1ng/ml\n\nPrecision:пїЅ\nIntra-assay error: <5%\nInter-assay error: <14%\n\nRange: 0-100ng/ml\n\nLinear Range: 0.10-1.05ng/ml\n\nSpecificity:\nPeptide/% Crossreactivity\nGhrelin (Rat) 100\nGhrelin (Human)100\nGhrelin (Des-Octanoyl-Ser3) (Human)100\nGhrelin (Des-Octanoyl-Ser3) (Rat) 100\nC-Terminal of Ghrelin 100\nGhrelin (Porcine) 0\nGhrelin (1-14) 0\nGhrelin (1-5) 0\nSecretin (Human) 0\nVIP (Human, Porcine, Rat) 0\nProlactin-Releasing Peptide-31 (Rat) 0\nProlactin-Releasing Peptide-31 (Human) 0\nGalanin (Rat) 0\nGalanin (Human) 0\nGHRF (Rat) 0\nGHRF (Human) 0\nNPY (Human Rat) 0\nOrexin A (Human Bovine Mouse Rat) 0\nOrexin B (Human) 0\nOrexin B (Mouse, Rat) 0\nbeta-Endorphin (Rat) 0\nbeta-Endorphin (Human) 0\nalpha-MSH 0\nMCH 0\n\nStorage and Stability: \nStore components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.пїЅ \n

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SPECIFICATIONS

Catalog Number

G2033-73

Size

1Kit

Applications

ELISA

Reactivities

Mouse, Rat

References

1. Enzyme Immunoassay Techniques, An Overview. Porstmann, T. and Kiessig, S.T. Journal of Immunological Methods, 150(1992) 5-21. 2. Amplification Systems in Immunoenzymatic Techniques. Avrameas, S. Journal of Immunological Methods. 150(1992) 23-32. 3. Shuto, et al. Journal of Clinical Investigation. 109 (2002) 1429-1436.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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