Gi alpha 1,2

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000 dilution detected the Gia-1 and Gia-2 protein in 40ug rat brain microsomal preparation and in 10ug of solubilized plasma membranes from mouse 3T3/A31 fibroblasts. The rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with G2033-90 (1:1000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.\nImmunocytochemistry: 1:500 dilution gave positive immunostaining of mouse 3T3/A31 fibroblasts fixed for 3 minutes with ice-cold 100% acetone.\nOptimal dilution determined by the researcher. \n\nRecommended Secondary Antibodies\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb\nI1904-46Q: IgG, H&L (HRP) Pab Gt xRb\nI1904-40D: IgG, H&L (FITC) Pab Gt xRb\nI1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb \n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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