Gi alpha 3

Gi alpha 3 is a G-protein subunit that is involved in many signal transduction pathways including the mediation of EGF-induced PLC-gamma activation and Ca+2 mobilization in hepatocytes1,2. G-proteins are membrane associated heterotrimeric proteins that are comprised of alpha, beta-, and lambda-subunits. The alpha-subunit contains a guanine-binding domain that is in its inactive state when it is occupied by GDP. Upon activation, GDP is replaced with GTP, causing the dissociation of the alpha-subunit from the beta, lambda-subunit complex. This enables the G alpha-GTP complex to bind to and regulate specific signaling pathways. GTP is then hydrolyzed, allowing for re-association of the alpha-subunit with the beta, lambda- subunit complex.\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000-1:2000 detected Gi alpha 3 in rat hippocampus membrane preparation. Previous lots detected Gi apha 3 in solubilized rat and mouse brain membranes and mouse 3T3/A31 cell lysates.\nImmunoprecipitation: 4ul immunoprecipitated Gi alpha 3 from a solubilized mouse brain membrane lysate.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G2033-91

Size

50ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Lynch, C J, et al (1989). J Clin Invest 83: 2050-62. 2. Barrett, P., et al (2000). A role for tyrosine phosphorylation in the regulation and sensitization of adenylate cyclase by melatonin. Faseb J 14: 1619-28. 3. Ward, D. T., et al (1998). Disulfide bonds in the extracellular calcium-polyvalent cation-sensing receptor correlate with dimer formation and its response to divalent cations in vitro. J Biol Chem 273: 14476-83. 4. Sands, J. M., et al (1997). Apical extracellular calcium/polyvalent cation-sensing receptor regulates vasopressin-elicited water permeability in rat kidney inner medullary collecting duct. J Clin Invest 99: 1399-405. 5. van Biesen, T., et al (1996). G(o)-protein alpha-subunits activate mitogen-activated protein kinase via a novel protein kinase C-dependent mechanism. J Biol Chem 271: 1266-9.

Additional Info

Recognizes Gi alpha 3 and Go alpha, Mr 40kD. Low crossreactivity with other G-proteins. Species Crossreactivity: human, mouse, hamster and rat