Giardiasisis a diarrhoeal illness caused by a single celled microscopic protozoan parasite, Giardia lamblia, also known as Giardia intestinalis. Giardia lamblia exists in two forms, an active form called a trophozoite, and an inactive form called a cyst. The active trophozoite attaches to the lining of the small intestine and is responsible for causing the signs and symptoms of giardiasis. The trophozoite cannot live long outside of the body and spread of infection is via the cyst which is excreted in the host's faeces. When it is ingested, stomach acid activates the cyst, and the cyst develops into the disease causing trophozoite in the new host. Giardiasis is diagnosed by finding cysts or trophozoites in the faeces.
Applications:
Suitable for use in Immunofluorescence and ELISA. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
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