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Git1 (ARF GTPase-activating protein GIT1)

Cat no: G2035-07Q

Git1 (ARF GTPase-activating protein GIT1)

ARF GTPase-activating protein GIT1 (Git1) is a multidomain protein belonging to the ARF family of small GTPases that is comprised of several protein interacting domains including: three Ankyrin repeats (ANKs), an Arf-Gap domain, a C-terminal GRK-binding domain, and a spa2 homology domain. Git1 is thought to have a role as a signaling pathway coordinator, functioning to regulate adhesion, cytoskeletal organization, and vesicle trafficking. It is also a component of several cytoplasmic complexes that contain ARHGEF6, PAK1, and PXN. In brain receptors, Git1 forms a complex with AMPA receptors, GRIP, and liprin-alpha, that is crucial to AMPA receptor targeting and the organization of synapses. Git1 expression is observed in a wide variety of tissues, with the most notable expression in the testis.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. \n\nRecommended Dilutions: \nWestern Blot: 0.5ug/ml detects Git1 on 10ug of rat brain membrane tissue lysate.\nOptimal dilutions to be determined by the researcher. \n\nPositive Control: \nRat brain membrane tissue lysate.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G2035-07Q

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

Additional Info

Recognizes rat Git1.

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