GITRL (Tumor Necrosis Factor Ligand Superfamily Member 18, Activation-inducible TNF-related Ligand, AITRL, Glucocorticoid-induced TNF-related Ligand, hGITRL, TNFSF18, AITRL, GITR, TL6, UNQ149/PRO175) (FITC)

GITR or TNFRSF18 is a 241aa novel member of the tumor necrosis factor receptor superfamily and a receptor for TNFSF18. It has three extracytoplasmic cysteine-rich pseudorepeats, a small cytoplasmic tail and a DEATH domain. It is an essential regulator of immune responses to self tissues and pathogens and plays a key role in dominant immunological self-tolerance maintained by CD25 (+) CD4 (+) regulatory T cells. It is known to be involved in regulation of CD3-driven T-cell activation and programmed cell death and also in interactions between activated T-lymphocytes and endothelial cells. In mammalian cells it mediates NF-kappa-B activation via TRAF2 and modulates T lymphocyte survival in peripheral tissues. It is mostly expressed in lymph node, peripheral blood leukocytes and weakly expressed in spleen. GITR and its ligand are also known to be involved in atopic dermatitis.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry (Cell Surface): 0.1-0.3ug/10e6 cells\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

G2035-09N2

Size

100ug

Applications

FC

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in 10mM Tris, 150mM sodium chloride, 0.05% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b,l

References

1. Esparza, EM. et al. J. Immonol. 174:7875-7882 (2005). 2. Bushell, A. et al. Am. Transplant. 7:759-768 (2007). 3. Kwon, B. et al. J. Biol. Chem. 274:6056-6061 (1999).

Additional Info

Recognizes mouse GITRL.