GLi1 (Glioma-associated Oncogene, Kruppel (Kr) Zinc Finger Protein)

GLI was first identified as a gene amplified in a malignant glioma (1) and can transform primary cells in cooperation with adenovirus E1A (2). GLI belongs to the Kruppel family of zinc finger proteins and there are three GLI proteins in mammals: GLI1, GLI2 and GLI3 (3). GLI proteins, similar to their Drosophila homolog Ci (Cubitus interruptus), function as transcription factors activated by the Hedgehog signaling pathway which plays an important role in animal development and is aberrantly activated in many types of cancers (4,5).\nGLI1 itself is a transcriptional target of the Hedgehog signaling pathway (6-8) and is used as a marker of Hedgehog signaling activation in cancer research (9,10).\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.\n\nRecommended Dilution:\nImmunoprecipitation: 1:50\nWestern Blot: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G2035-51

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol and less than 0.02% sodium azide.

P Type

Pab

Purity

Purified by Protein A and immunoaffinity chromatography.

References

1 Kinzler, K.W. et al. (1987) Science 236, 70-73. 2 Ruppert, J.M. et al. (1991) Mol. Cell Biol. 11, 1724-1728. 3 Kinzler, K.W. et al. (1988) Nature 332, 371-374. 4 Ingham, P.W. and McMahon, A.P. (2001) Genes Dev. 15, 3059-3087. 5 McMahon, A.P. et al. (2003) Curr. Top. Dev. Biol. 53, 1-114. 6 Lee, J. et al. (1997) Development 124, 2537-2552. 7 Dahmane, N. et al. (1997) Nature 389, 876-881. 8 Dai, P. et al. (1999) J. Biol. Chem. 274, 8143-8152. 9 Watkins, D.N. et al. (2003) Nature 422, 313-317. 10 Karhadkar, S.S. et al. (2004) Nature 431, 707-712,

Additional Info

Detects endogenous levels of total human GLI1 protein.