GLI1 (Zinc Finger Protein GLI1, Glioma-associated Oncogene, Oncogene GLI, GLI)

GLI1 is a protein originally isolated in human glioblastoma. The GLI proteins are the effectors of Sonic Hedgehog (Hh) signaling and have been shown to be involved in cell-fate determination, proliferation and patterning in many cell types and most organs during embryo development. GLI1 was originally isolated from a glioma tumor and has been found to be up regulated in many tumors including muscle, brain and skin tumors such as Basal cell carcinoma (BCC). Shh and the Gli genes are normally expressed in hair follicles, and skin tumors expressing Gli1 may arise from hair follicles. The level of Gli1 expression correlates with the tumor grade in bone and soft tissue sarcomas.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-3ug/ml\nImmunohistochemistry (Formalin fixed paraffin): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G2035-51C

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Dahmane, N. et al. Nature 389:876-881 (1997). 2. Callahan, C. A. et al. Genes Dev. 18:2724-2729 (2004). 3. Dunaeva, M. et al. J. Biol. Chem. 278:5116-5122 (2003).

Additional Info

Recognizes human GLI1. Species Crossreactivity: rhesus monkey. Species Sequence Homology: equine (94%), canine (88%), bovine, mouse and rat (76%)