GLI2 (GLI-Kruppel Family Member 2, GLI-Kruppel Family Member GLI2, GLI Family Zinc Finger 2, HPE9, Tax Helper Protein, THP, THP1, THP2, Zinc Finger Protein GLI2)

GLI2, also known as GLI-Kruppel family member 2, a 133.3kDa protein belonging to the C2H2-type zinc finger protein subclass of the Gli family of transcription factors. GLI2 and the related GLI3 proteins are the primary transcription factors that mediate Sonic hedgehog (Shh) signals in the mouse, GLI2 being primarily a transcriptional activator while GLI3 is a transcriptional repressor. Studies indicate that GLI2 plays a role in embryogenesis. GLI proteins are also implicated as potent oncogenes in the embryonal carcinoma cell.\n\nApplications:\nSuitable for use in ELISA, Immunohistochemistry, and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry (Paraffin): 5ug/ml. Requires antigen retrieval using heat treatment for 20 minutes prior to staining of paraffin sections. 0.01M sodium citrate buffer, pH 6 is recommended.\nELISA: 1:15,000-1:60,000\nWestern Blot: 1:500-1:2000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control for Immunohistochemistry:\nMouse brain\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G2035-53D

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

Additional Info

Recognizes mouse GLI2. Species Crossreactivity: rat

Alternative Names

Oncogene GLI2, Tax Helper Protein 1, Tax Helper Protein 2, Tax Responsive Element 2 Holding Protein, Tax Responsive Element-25 bp Sequence Binding Protein