Gliadin (Celiac Disease)

Celiac disease is associated with a CD4+ T-cell response to epitopes of gliadin presented by HLA-DQ2 or -DQ8 class II MHC molecules. These epitopes are present in a 33-mer peptide of wheat alpha-gliadin, residues 56-88, which is resistant to digestion and forms a substrate for tissue transglutaminase (TG2), generating the glutamic acid residues essential for binding to HLA-DQ2. The immunogen corresponds to a deamidated form of a region that includes the T-cell epitopes, including the immunodominant PQPQLPY region and two PXPQP motifs associated with binding to IgA from patients with celiac disease. Complete homology exists between residues 63-73, 70-80 and 77-87 of wheat alpha gliadin.\n\nApplications: \nSuitable for use in ELISA (1). Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:64,000 (~20-fold higher concentration is needed to recognize non-deamidated peptide.)\nOptimal dilutions to be determined by the researcher.\n\nCulture Medium:\nRPMI 1640 with 2-10% fetal calf serum\n\nHybridoma: \nX63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G2035-85

Size

100ul

Applications

ELISA

Hosts

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG2a,k

References

1. Skovbjerg H, et al. Biochim Biophys Acta 1690:220-230.(2004). 2. Mowat AM., Lancet 361:1290-2.(2003)

Additional Info

Recognizes Gliadin. Reacts with the deamidated gliadin peptide (KLQPFPQPELPYPQPQ). Weakly crossreacts (5%) with the\nnon-deamidated gliadin peptide (KLQPFPQPELPYPQPQ).