Gliadin IgA BioAssay(TM) ELISA Kit

Gliadin IgA ELISA test system is designed to detect IgA class antibodies to Gliadin in human sera. Wells of plastic microwell strips are sensitized by passive absorption with Gliadin antigen. The test procedure involves three incubation steps:\n1. Test sera (properly diluted) are incubated in antigen coated microwells. Any antigen specific antibody in the sample will bind to the immobilized antigen. The plate is washed to remove unbound antibody and other serum components.\n2. Peroxidase Conjugated goat anti-human IgA is added to the wells and the plate is incubated. The Conjugate will react with Gliadin antibody immobilized on the solid phase in step 1. The wells are washed to remove unreacted Conjugate.\n3. The microwells containing immobilized peroxidase Conjugate are incubated with peroxidase Substrate Solution. Hydrolysis of the Substrate by peroxidase produces a color change. After a period of time the reaction is stopped and the color intensity of the solution is measured photometrically. The color intensity of the solution depends upon the antibody concentration in the original test sample.\n\nSpecificity:\n96%\n\nSensitivity: \n93.90%\n\nKit Components:\nMicrotiter plate, 1x96 wells \nConjugate (HRP) Reagent, 1x15ml\nPositive Control (Human Serum), 1x350ul\nCalibrator (Human Serum), 1x500ul\nNegative Control (Human Serum), 1x350ul\nSample Diluent, 1x30ml\nTMB Substrate, 1x15ml \nStop solution, 1x15ml\nWash Buffer (10X), 1x100ml\n\nStorage and Stability:\nStore at 4 degrees C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

167553

Size

96Tests

Applications

ELISA

References

1. Trocone R, Ferguson A: Anti-gliadin Antibodies. J. of Ped. Gastro and Nut. 12:150-158, 1991. 2. Swinson CM, Levi AJ: Is coeliac disease underdiagnoses? BMJ 281:1258-1260, 1980. 3. Logan RFA, tucker G, Rifkind EA, Heading RC,Ferguson A: Changes in clinical features of coeliac disease in adults in Edinburgh and the Lothianss 1960-79. BMJ 286:95-97, 1983. 4. Maki M, Kallonen K, Lahdeaho ML, Visakorpi JK: Changing pattern of childhood coeliac disease in Finland. Acta Paediar Scand 77:408-412, 1988. 5. Procedures for the Handling and Processing of Blood Specimens: NCCLS Procedure H18. Approved guideline. 6. Procedures for the collection of diagnostic blood specimens by venipuncture: NCCLS Procedure H3, Approved Standard. 7. U.S. Department of Labor (OSHA):Occupational Exposure to Bloodborne Pathogens. Final Rule. 21CFR\n1910.1030.