Glial Derived Neurotrophic Factor (GDNF) Recombinant, Human

Supplier:

United States Biological

Cat no: G2036-02B

Glial Derived Neurotrophic Factor (GDNF) Recombinant, Human

This factor was found initially in conditioned medium of the rat B49 glial cell line. GDNF is a disulfide-bonded homodimeric glycosylated protein of 134 amino acids. The proteins contain seven conserved cysteine residues in the same relative spacing found in all members of the TGF-beta superfamily of proteins. The human GDNF gene has been mapped to chromosome 5p13.1-p13.3. Related factors are Neurturin, Persephin, and Artemin. Collectively they are being referred to as GDNF family ligands.\n\nEndotoxin: <0.01ng/ug or <0.1EU/ug as per LAL\n\nAA Sequence:\nMSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI\n\nBiological Activity: Glial-Derived Neurotrophic Factor is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent dopamin uptake in rat mesencephalic cultures was found to be 5-10ng/ml.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G2036-02B

Size

2ug

Form

Supplied as a lyophilized powder. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.

Purity

(same/more than) 98% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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