Glial fibrillary acidic protein (GFAP) is an intermediate filament protein found in glial cells such as astrocytes and ependymal cells. GFAP is also seen in Schwann cells, enteric glial cells and satellite cells of the sensory ganglia in the peripheral nervous system. This antibody will help detect GFAP and identify these cell types in normal and uncharacterized tissues.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry: 1:100 for 1 hr at RT
Optimal dilutions to be determined by the researcher.
Positive Control:
Brain. Cytoplasmic staining pattern.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.