Glucagon BioAssay(TM) ELISA Kit (Human, Mouse, Rat)

Supplier:

United States Biological

Cat no: 167837

Glucagon BioAssay(TM) ELISA Kit (Human, Mouse, Rat)

Glucagon Enzyme Immunoassay (EIA) BioAssay(TM) Kit is an in vitro quantitative assay for detecting Glucagon peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- Glucagon antibody, both biotinylated Glucagon peptide and peptide standard or targeted peptide in samples interacts competitively with the Glucagon antibody. Uncompeted (bound) biotinylated Glucagon peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Glucagon peptide in the standard or samples. This is due to the competitive binding to Glucagon antibody between biotinylated Glucagon peptide and peptides in standard or samples. A standard curve of known concentration of Glucagon peptide can be established and the concentration of Glucagon peptide in the samples can be calculated accordingly. The minimum detectable concentration of Glucagon is 0.97pg/ml. \n\nSample Type:\nSerum, plasma and cell culture media etc.\n\nIntended Use:\nThe BioAssay(TM) kit is used for quantitative protein detection, establishing normal range and validation of antibody array results.\n\nSpecificity:\nThe capture antibody provided in this kit recognizes human, mouse and rat Glucagon.\n\nSensitivity:\nThe intra-assay reproducibility is CV<10% and inter-assay is CV<12%. \n\nRange:\nThe detection range for the kit is 1-1000pg/ml\n\nKit Components:\nGlucagon Microplate (Item A), 1x96wells\nWash Buffer Concentrate (Item B), 1x25ml\nLyophilized standard Glucagon peptide (Item C), 2x vials\nLyophilized biotinylated Glucagon peptide (Item F), 2x vials\nHRP-Streptavidin concentrate (Item G), 1x600ul\nTMB One-Step Substrate Reagent (Item H), 1x12ml\nStop Solution (Item I), 1x8ml\nLyophilized positive control (Item M), 1x vial\nLyophilized anti-Glucagon polyclonal antibody (Item N), 2x vials\n1X Assay Diluent E (Item R), 2x vials\n\nStorage and Stability:\nStore powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

167837

Size

100Tests

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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