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Glucagon (GCG) ELISA Kit

Glucagon (GCG) ELISA Kit

Cat no: ABIN1873470

Supplier: Antibodies-online
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Glucagon (GCG) ELISA Kit: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to GLP2 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled GLP2 and unlabeled GLP2 (Standards or samples) with the pre-coated antibody specific to GLP2. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of GLP2 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of GLP2 in the sample.
Catalogue number: ABIN1873470
Size: 96 tests
Antigen: Glucagon Like Peptide 2 (GLP2)
Sensitivity: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Assay range: 123.46-10000 pg/mL
Sample type: Serum,Plasma,Tissue Homogenate,Cell Lysate,Cell Culture Supernatant,Biological Fluids

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