Glucagon, Human (GLP1, GLP2, GRPP)

Human Synthetic Glucagon is a single, non-glycosylated, polypeptide chain containing 29 amino acids and having a molecular mass of 3483 Dalton. Glucagon is beneficial for the culture of some cell types. It has been used in some biochemical regulation studies of glycogenolysis in hepatocytes. It has been also been found to induce DNA replication in primary cultures of adult rat hepatocytes when used in combinations with EGF and Insulin. Glucagon increases the blood glucose concentration by promoting rapid breakdown of liver glycogen, and also acts to relax smooth muscle such as the gastrointestinal tract.\n\nAmino Acid Sequence: H-His-Ser-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-OH\n\nStorage and Stability: \nLyophilized powder may be stored at -20 degrees C. Reconstitute to (same/more than)100ug/ml with sterile ddH2O. Reconstituted product is stable for 6 months at -20 degrees C. For long term storage add 0.1% HSA or BSA, aliquot, and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G2040-01G

Size

4mg

Form

Supplied as a lyophilized powder without additives. Reconstitute in sterile dH2O to a concentration (same/more than)0.1mg/ml. Can be further diluted to other aqueous solutions.

Purity

(same/more than) 98% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Transcription factor FoxO1 mediates glucagon-like peptide-1 effects on pancreatic beta-cell mass. Diabetes 2006 May;55(5):1190-6 2. Homotypic cell contact enhances insulin but not glucagon secretion. Biochem Biophys Res Commun 2006 Jun 9;344(3):995-1000 3. The role of glucagon in regulating chicken hepatic malic enzyme and histidase messenger ribonucleic acid expression in response to an increase in dietary protein intake.Poult Sci 2006 Apr;85(4):753-60 4. Islets transplanted intraportally into the liver are stimulated to insulin and glucagon release exclusively through the hepatic artery.Am J Transplant 2006 May;6(5 Pt 1):967-755. Blockade of GABA(A) receptors in the ventromedial hypothalamus further stimulates glucagon and sympathoadrenal but not the hypothalamo-pituitary-adrenal response to hypoglycemia. Diabetes 2006 Apr;55(4):1080-76. Postprandial suppression of glucagon secretion depends on intact pulsatile insulin secretion: further evidence for the intraislet insulin hypothesis.Diabetes 2006 Apr;55(4):1051-6