Glucagon-like Peptide 2 (GLP2)

Glucagon is a member of a multigene family comprising of Secretin, Vasoactive Intestinal Peptide (VIP), Gastric Inhibitory Peptide (GIP) and others like Glicentin and Oxyntomodulin (OXM), which differs from glucagon by C-terminal octapeptide. The glucagon precursor contains at least 3 intervening sequences that divide the protein-coding portion into 4 regions corresponding to the signal peptide and part of the N-terminal peptide, the remainder of the N-terminal peptide and glucagon, glucagon-like peptide-1 (GLP1), and GLP2. The GLP 1 & 2 stimulates intestinal growth and up regulates villus height in the small intestine, concomitant with increased crupt cell proliferation and decreased enterocyte apoptosis. The two GLP's are mainly produced in the A cells of the Islets of Langerhans in response to a drop in blood sugar concentration. GLP2, also a processed active peptide with 33aa, (chr 2q36-q37). GLP2 regulates gastric motility, gastric acid secretion, intestinal hexose transport, and increases the barrier function of the gut epithelium. It significantly enhances the surface area of the mucosal epithelium via stimulation of crypt cell proliferation. The actions of GLP2 are transduced by the GLP2 receptor, a G-protein-coupled receptor, activation of receptor signaling in heterologous cells promotes resistance to apoptotic injury in vitro, as such it may potentially be useful in treatment of injury and dysfunction of intestinal mucosal epithelium.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml using Chemiluminescence.\nELISA: 1:10,000-1:100,000; using 50-100ng G2040-37A control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G2040-37

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, GP

Form

Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Suzuki A et al, PNA ( April 2003); Yamamot H et al, J Neurosci (2003) 23(7) 2939-46; 2. Yves Rouille, JBC (1995) 270 : 26488-96.

Additional Info

Recognizes human GLP2. Species sequence homology: 100% in mouse, rat, guinea pig and 88% in bovine. No significant sequence homology is detected with other proteins.