Glucocorticoid Receptor (GCCR, GCR, Glucocorticoid receptor lymphocyte, GR, GRL, Grl1 NR3C1, Nuclear receptor subfamily 3 group C member 1)

Cellular Localization: Cytoplasmic in the absence of ligand; nuclear after ligand-binding.\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 5ug/ml\nImmunohistochemistry-Paraffin 1:10-1:2000\nImmunocytochemistry/Immunofluorescence 1ug/ml\nImmunohistochemistry 1:10-1:2000 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G2040-51B

Size

50ug

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

This product has been used in:Cidlowski JA et al. Novel antipeptide antibodies to the human glucocorticoid receptor: recognition of multiple receptor forms in vitro and distinct localization of cytoplasmic and nuclear receptors. Mol Endocrinol 4:1427-37 (1990). <PUBMED:1704480> Whitelaw ML et al. Heat shock protein hsp90 regulates dioxin receptor function in vivo. Proc Natl Acad Sci U S A 92:4437-41 (1995). <PUBMED:7753824>van Steensel B et al. Localization of the glucocorticoid receptor in discrete clusters in the cell nucleus. J Cell Sci 108 (Pt 9):3003-11 (1995). <PUBMED:8537440>Gaitan D et al. Glucocorticoid receptor structure and function in an adrenocorticotropin-secreting small cell lung cancer. Mol Endocrinol 9:1193-201 (1995). <PUBMED:7491111>Whitelaw ML et al. Identification of transactivation and repression functions of the dioxin receptor and its basic helix-loop-helix/PAS partner factor Arnt: inducible versus constitutive modes of regulation. Mol Cell Biol 14:8343-55 (1994). <PUBMED:7969169>Trapp T et al. Heterodimerization between mineralocorticoid and glucocorticoid receptor: a new principle of glucocorticoid action in the CNS. Neuron 13:1457-62 (1994). <PUBMED:7993637>Bellingham DL et al. Ligand-dependent down-regulation of stably transfected human glucocorticoid receptors is associated with the loss of functional glucocorticoid responsiveness. Mol Endocrinol 6:2090-102 (1992). <PUBMED:1491690>Rangarajan PN et al. Modulation of glucocorticoid receptor function by protein kinase A. Mol Endocrinol 6:1451-7 (1992). <PUBMED:1435789>Burnstein KL et al. Autoregulation of glucocorticoid receptor gene expression. Steroids 56:52-8 (1991). <PUBMED:2020978>McGimsey WC et al. Immunocytochemical localization of the glucocorticoid receptor in rat brain, pituitary, liver, and thymus with two new polyclonal antipeptide antibodies. Endocrinology 129:3064-72 (1991). <PUBMED:1720088>Burnstein KL et al. Human glucocorticoid receptor cDNA contains sequences sufficient for receptor down-regulation. J Biol Chem 265:7284-91 (1990). <PUBMED:1692020>

Additional Info

Recognizes Glucocorticoid Receptor at ~97kD from rat liver extract. Species Crossreactivity: Crossreacts with Human, Rat and Mouse. Not yet tested in other species.

Alternative Names

GCCR, GCR, Glucocorticoid receptor lymphocyte, GR, GRL, Grl1 NR3C1, Nuclear receptor subfamily 3 group C member 1