Glucocorticoid Receptor (GCCR, GCR, Glucocorticoid receptor lymphocyte, GR, GRL, Grl1 NR3C1, Nuclear receptor subfamily 3 group C member 1)

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor. The corresponding gene for the glucocorticoid receptor is NR3C1.\n\nApplications: \nSuitable for use in Flow Cytometry, Immunocytochemistry, Gel Super Shift, Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:10-1:1000\nImmunocytochemistry (IF): 1:10-1:200\nGel Super Shift: 1:1-1:100\nWestern Blot: 5ug/ml\nImmunoprecipitation: 1:10-1:500\nImmunohistochemistry: 1:10-1:2000\nImmunohistochemistry (paraffin): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nL929 cells and mouse liver extract\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G2040-51C

Size

100ug

Applications

FC, ICC, IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, GP, Rabbit, Sheep

Form

Supplied as a lyophillized powder from PBS, pH 7.2, 0.05% sodium azide. Reconstitute in 100ul of sterile dH2O.

P Type

Mab

Purity

Purified

Isotype

IgG2

References

Wyszomierski SL et al. Mol Endocrinol 13:330-43 (1999). <PUBMED:9973262>Barent RL et al. Mol Endocrinol 12:342-54 (1998). <PUBMED:9514152>Dao-Phan HP et al. Mol Endocrinol 11:962-72 (1997). <PUBMED:9178755>Dittmar KD & Pratt J Biol Chem 272:13047-54 (1997). <PUBMED:9148915>Chang HC et al. Mol Cell Biol 17:318-25 (1997). <PUBMED:8972212>Sackey FN et al. Mol Endocrinol 10:1191-205 (1996). <PUBMED:9121487>Renoir JM et al. Proc Natl Acad Sci U S A 92:4977-81 (1995). <PUBMED:7539138>Nordeen SK et al. Endocrinology 136:1120-7 (1995). <PUBMED:7532578>Berghorn KA et al. Endocrinology 136:804-7 (1995). <PUBMED:7835313>Opoku J & Simons SS J Biol Chem 269:503-10 (1994). <PUBMED:8276843>Hutchison KA et al. J Biol Chem 269:27894-9 (1994). <PUBMED:7961721>Stancato LF et al. R J Biol Chem 268:21711-6 (1993). <PUBMED:8408024>Chang TJ et al. Mol Endocrinol 7:528-42 (1993). <PUBMED:8502237>Schlatter LK et al. Mol Endocrinol 6:132-40 (1992). <PUBMED:1738366>Hutchison KA et al. J Biol Chem 267:2902-8 (1992). <PUBMED:1310678>Distelhorst CW & Howard KJ J Steroid Biochem 36:25-31 (1990). <PUBMED:2362447>Raaka BM et al. Mol Endocrinol 3:332-41 (1989). <PUBMED:2710136>Orti E et al. J Biol Chem 264:231-7 (1989). <PUBMED:2909517>Distelhorst CW & Howard KJ J Biol Chem 264:13080-5 (1989). <PUBMED:2753901>Smith LI et al. Biochemistry 28:4490-8 (1989). <PUBMED:2765497>Unger AL et al. Mol Endocrinol 2:952-8 (1988). <PUBMED:2460750>Bresnick EH et al. J Steroid Biochem 30:267-9 (1988). <PUBMED:3386251>Hendry WJ et al. Endocrinology 120:629-39 (1987). <PUBMED:3542507>Gametchu B & Harrison RW Endocrinology 114:274-9 (1984). <PUBMED:6690272> Yoshinaga SK et al. Science 258:1598-604 (1992). <PUBMED:1360703>Chadwick CC et al. J Biol Chem 267:3473-81 (1992). <PUBMED:1371119>Bresnick EH et al. Biochemistry 29:520-7 (1990). <PUBMED:2405907>LaFond RE et al. Exp Cell Res 175:52-62 (1988). <PUBMED:3345802>Rehmus EH et al. J Steroid Biochem 28:167-77 (1987). <PUBMED:3650602>

Additional Info

Recognizes the undigested 97kD rat GR, a 17kD DNA-binding trypsin fragment, and a 45kD steroid- and DNA-binding chymotrypsin fragment. Immunocytochemistry staining of GR in L929 cells with this antibody results in staining of both the cytoplasm and nucleus, even in the presence of hormone. Species Crossreactivity: Guinea pig, mouse, rabbit and sheep. Does not crossreact with Amphibians, birds or primate.

Alternative Names

GCCR, GCR, Glucocorticoid receptor lymphocyte, GR, GRL, Grl1 NR3C1, Nuclear receptor subfamily 3 group C member 1