Glucose-6-Phosphate Dehydrogenase (G6PDH) BioAssay(TM) Kit

Supplier:

United States Biological

Cat no: G3053-25B

Glucose-6-Phosphate Dehydrogenase (G6PDH) BioAssay(TM) Kit

Glucose-6-phosphate dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Of greater quantitative importance is the production of NADPH for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as the liver, mammary glands, adipose tissue, and the adrenal glands. The glucose-6-phosphate dehydrogenase Assay Kit is a simple, sensitive and rapid assay detects the activity of G6PDH in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product which is utilized to convert a nearly colorless probe to an intensely colored product with an absorbance at 450nm. The G6PDH Assay Kit can detect as low as 0.04mU G6PDH per well.\n\nKit Components:\nG6PDH *Assay Buffer 1x25ml\nG6PDH Substrate lyophilized\nG6PDH Developer lyophilized\nG6PDH Positive Control lyophilized\nNADH Standard (0.5umol) lyophilized.\n\nStorage and Stability:\nStore the kit at -20 degrees C, protect from light. Allow *Assay Buffer to warm to RT before use. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G3053-25B

Size

100Tests

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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