Glucose Assay Kit, BioAssay(TM), Colorimetric/Fluorimetric

Glucose (C6H12O6) is a key diagnostic parameter for many metabolic disorders. Increased glucose levels have been associated with diabetes mellitus, hyperactivity of thyroid, pituitary and adrenal glands. Decreased levels are found in insulin secreting tumors, myxedema, hypopituitarism and hypoadrenalism. Simple, direct and high-throughput assays for measuring glucose concentrations find wide applications in research and drug discovery. Glucose assay kit uses a single Working Reagent that combines the glucose oxidase reaction and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at lem/ex=585/530nm is directly proportional to glucose concentration in the sample.\n\nKey Features:\nSensitive and accurate. Use as little as 20ul samples. Linear detection range in 96-well plate: 5 to 300uM (90 ug/dL to 5.4 mg/dL) glucose for Colorimetric Assay:s and 1 to 30uM for Fluorimetric Assay:s.\nSimple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature.\n\nApplications:\nDirect Assays: glucose in serum, plasma, urine, saliva, milk, culture medium and other biological samples.\nDrug Discovery/Pharmacology: effects of drugs on glucose metabolism.\nFood and Beverages: glucose in food, beverages etc.\n\nKit Contents:\nAssay Buffer: 10ml Enzyme Mix: 120ul\nDye Reagent: 120ul Standard: 1ml 300 mg/dL Glucose\nStorage conditions. The kit is shipped on dry ice. Store Assay Buffer at 4 degrees C and other components at -20 degrees C. Shelf life: 6 months after receipt.\nPrecautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.\n\nColorimetric Procedure:\nSample treatment: saliva samples should be centrifuged for 5 min at 14,000 rpm prior to assay. Milk samples should be cleared by mixing 100ul 6N HCl and 600ul milk. Centrifuge 5 min at 14,000 rpm and transfer supernatant into a clean tube. Add 170ul 6N NaOH perml supernatant. Mix well and centrifuge again at 14,000 rpm. The supernatant can be assayed. The dilution factor in this procedure is n=1.36. Samples can be analyzed immediately after collection, or stored in aliquots at

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SPECIFICATIONS

Catalog Number

G3042-04

Size

1Kit

References

1. Okuda J, Okuda G. (1969). A rapid polarographic microdeter- mination of glucose with glucose oxidase. Clin Chim Acta. 23(2): 365-7.\n2. Saifer A, Gerstenfeld S. (1958). The photometric microdeter- mination of blood glucose with glucose oxidase. J Lab Clin Med. 51(3): 448-60.\n3. Middleton JE, Griffiths WJ. (1957). Rapid colorimetric micro-method for estimating glucose in blood and C. S. F. using glucose oxidase. Br Med J. 2(5060): 1525-7.