Glucose Oxidase Activity, Assay Kit, BioAssay(TM) (Beta-D-glucose:oxygen 1-oxido-reductase, Glucose Oxyhydrase, GOD, GOX)

The glucose oxidase enzyme (GOx) (EC 1.1.3.4) is an oxido-reductase that catalyzes the oxidation of glucose to hydrogen peroxide and D-glucono-d-lactone. In cells, it aids in breaking the sugar down into its metabolites. USBio's Glucose Oxidase Assay Kit provides a convenient tool for sensitive detection of the Glucose Oxidase in a variety of samples. The Glucose Oxidase in sample will recognize d-glucose as a specific substrate leading to proportional color development. The activity of GOx can be easily quantified colorimetrically (570nm) or fluorometrically (Ex/Em = 535/587). This assay detects GOx activity as low as 0.01mU with our unit definition.\n\nSample Type:\nCells and biological fluids\n\nIntended Use:\nFor sensitive detection of the Glucose Oxidase in a variety of samples.\n\nSensitivity:\n0.01mU\n\nSpecificity:\nProkaryotes, Archaea, etc.\n\nTest Principle:\nThe Glucose Oxidase in sample will recognize d-glucose as a specific substrate leading to proportional color development. The activity of GOx can be easily quantified colorimetrically (570nm) or fluorometrically (Ex/Em = 535/587).\n\nKit Components:\nGOx Assay Buffer: 1x28ml\nOxiRed(TM) Probe (in DMSO): 1x200ul\nGOx Substrate: 1x1ml\nDeveloper (lyophilized): 1 vial\nGOx Positive Control (lyophilized): 1 vial\nH2O2 Standard (0.88M): 1x100ul\n\nStorage and Stability:\nStore powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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