Glucose Oxidase (Aspergillus niger) (Biotin)

Since its discovery as an "antibiotic" (shown subsequently to be due to peroxide formation) there has been an interest in glucose oxidase, chiefly because of its utility in glucose estimation. For most clinical work the crude form of the A. niger enzyme has been satisfactory. However, it contains trace amount of polysaccharidases such as amylase, maltase and sucrase which can contribute to falsely high glucose levels. The purified enzyme is free of these traces and is recommended for analytical use in the presence of di-or polysaccharides. Glucose analytical systems employ the enzyme utilizing H2O2 production reacting with Fe(CN)-in the presence of luminol to produce luminescence proportional to the initial glucose concentration.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:5000-1:25,000. Assayed against 1ug of Glucose Oxidase in a standard capture ELISA using Streptavidin (HRP) (S7973-97A) and ABTS (A0030) as a substrate for 30 minutes at RT.\nWestern Blot: 1:500-1:3000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G3052-07

Size

1mg

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Bov

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with Biotin.

P Type

Pab

Purity

Purified by delipidation, salt fractionation and Ion Exchange chromatography.

Isotype

IgG

Additional Info

Recognizes Glucose Oxidase (Aspergillus niger). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified Glucose Oxidase (Aspergillus niger). Cross reactivity against Glucose Oxidase from other sources may occur but has not been specifically determined.