Glucose Oxidase (Aspergillus niger) (HRP)

Since its discovery as an "antibiotic" (shown subsequently to be due to peroxide formation) there has been an interest in glucose oxidase, chiefly because of its utility in glucose estimation. For most clinical work the crude form of the A. niger enzyme has been satisfactory. However, it contains trace amount of polysaccharidases such as amylase, maltase and sucrase which can contribute to falsely high glucose levels. The purified enzyme is free of these traces and is recommended for analytical use in the presence of di-or polysaccharides. Glucose analytical systems employ the enzyme utilizing H2O2 production reacting with Fe(CN)-in the presence of luminol to produce luminescence proportional to the initial glucose concentration.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:5000-1:25,000\nWestern Blot: 1:1000 -1:5000\nImmunohistochemistry: 1:500 -1:2500\nOptimal dilution determined by the researcher. \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

G3052-08

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Form

Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP). Reconstitute with 100ul sterile ddH2O.

P Type

Pab

Purity

Purified by Ion exchange chromatoghraphy.

Isotype

IgG

References

Farr & Nakane, J.Immunol. Methods 47:129-144. (1981).

Additional Info

Recognizes Glucose Oxidase (Aspergillus niger). Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti rabbit serum, as well as purified and partially purified Glucose Oxidase (Aspergillus niger).