Glucose Regulated Protein, 75kD (Grp 75)

Supplier:

United States Biological

Cat no: G3057-10

Glucose Regulated Protein, 75kD (Grp 75)

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. One class of stress proteins, termed the Hsp70 family, is comprised of multiple members, all of which bind ATP in vitro, but which are localized within different intracellular compartments. These include: i) Hsc70 (or constitutive form) present within the cytosol/nucleus; ii) Hsp70 (inducible form) present within the cytosol/nucleus/nucleolus; iii) the constitutive glucose-regulated 78kD (or BiP) protein present within the lumen of the endoplasmic reticulum; and iv) the constitutive glucose regulated 75kD protein present within the mitochondrial matrix. Members of the Hsp70 family are thought to funtion as molecular chaperones (2), assisting in the folding of other proteins in various intracellular compartments. Grp75 is localized in the mitochondrial matrix, where, in concert with Hsp60, is thought to participate in the re-folding of proteins translocated into this organelle. Like its E. coli homolog DnaK, Grp75 possesses a cation-dependent ATPase activity thought to be central to its function as a chaperone.\n\nSource:\nHT 1080 human fibrosarcoma cyplasmic lysate.\n\nApplications: \nSuitable for use in Western Blot. This preparation serves as the Grp 75 positive control and produces a readily visible band of Grp 75 when using chemiluminescent detection on Western Blots. Other applications not tested.\n\nRecommended Dilution:\nWestern Blotting: 30ul per lane.\n\nOptimum dilutions to be determined by researcher.\n\nStorage and Stability:\nStable for several weeks at 4 degrees C, several months at -20 degrees C, and indefinitely at -70 degrees C. Avoid repeated freeze/thaw cycles.

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SPECIFICATIONS

Catalog Number

G3057-10

Size

300ul

Applications

WB

Form

Supplied as a liquid.

References

1. Wadhwa, R., Kaul, S.C., Mitsui, Y., Cell Struct. Function, 19 : 1-10 (1994) 2. Kim, H.T., Nelson, Clayberger, Sanjanwala, M., Sklar, J., Krensky, A.M., J. Immunol., 154 : 1614-1623 (1995) 3. Mizzen, L.A., Chang, C., Garrels, J.I., Welch, W.J., Biol Chem., 264 : 20664-20675 (1989) 4. Webster, T.J., Naylor, D.J., Hartman, D.J., Hoj, P.B., Hoogenraad, N.J., DNA and Cell Biol., 13 : 1213-1220 (1994)

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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