Glucose Regulated Protein 78 (Grp78, BiP, KDEL)

The tetrapeptide, KDEL located at the carboxy-terminal sequences of luminal proteins is a retrieval motif essential for the precise sorting of these proteins along the secretory pathway. The KDEL proteins perform essential functions in the ER related to protein folding as well as assembly. The localization of chaperones and other soluble proteins to the endoplasmic reticulum (ER) is achieved by their continuous retrieval from post-ER compartments by the KDEL receptor (Erd2p) which is a membrane protein localized in the Golgi apparatus. The KDEL receptor has extensive sequence identity to the yeast Erd2p receptor for proteins bearing the tetrapeptide, HDEL, the yeast ER retention signal. Earlier studies using yeast mutants with deleted HDEL receptors had shown that this receptor was essential for retrieval of HDEL proteins and possibly for regulating transport through the Golgi complex. The regulation of transport through the KDEL receptor appears to be similar to many signal transduction processes in which membrane receptors act through a GTPase-activating protein (GAP that promotes hydrolysis of GTP to GDP) or a quanine nucleotide exchange factor (GEP that enhances exchange of GDP for GTP) of key small GTPases to regulate dfferent cellular functions. Recent data demonstrates that the non-catalytic domain of GAP that is essential for GAP activity in vivo, mediates the recruitment of cytosolic GAP to membranes and its interaction with the KDEL receptor. This interaction with the KDEL receptor is critical for GAP to act on ADP-ribosylation factor 1 (ARF1) which is a key regulator of transport in the secretory system.\n \nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Immunofluorescence and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot (Colormetric): 1:1000 \nImmunohistochemistry: Paraffin sections\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G3057-21

Size

50ug

Applications

ELISA, FC, ICC, IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Rat, Yeast/Fun

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Vaux, D., Tooze, J., Fuller, S.D. (1990) Nature 345: 495-502. 2. Pelham, H.R.B. (1990) Trends Biochem. Sci. 15: 483-486. 3. Scheel, A.A., Pelham, H.R.B. (1998) J. Biol. Chem. 273: 2467-2474. 4. Titorenko, V. I., Rachubinski, R.A. (1998) Mol. Cell. Biol. 18: 2789-2803. 5. Chouki, A., Ung, S., Wychowski, C., Dubuisson, J. (1998) J. Virology72: 3851-3858. 6. Wilson, B. S., Pfeiffer, J.R. Smith, A.J. Oliver, J.M. Oberdorf, J.A., Wojcikiewicz, R.J.H. (1998) Mol. Biol. Cell9: 1465-1478. 7. Paek, I., et. al. (1997) J. Cell Biol 137: 1017-1028. 8.Toyooka, K., Okamoto, T., Minamikawa, T. (2000) J. Cell Biol. 148: 453-463. 9. Paulsson, K.M., Wang, P., Anderson, P.O., Chen, S., Pettersson, R.F., Li, S. (2001) International Immunology. 13(8): 1063-1073. 10. Rubio, M.E.; Wenthold, R.J. (1999) J Neurochem 73(3): 942-948. 11. Kathuria, S., Sriraman, R., Nath, R., Sack, M., Pal, R., Artsaenko, O., Talwar, G.P., Fischer, R., Finnern, R. (2002).Hum Reprod 17(8): 2054-2061.

Additional Info

Recognizes rat Glucose Regulated Protein 78. Reacts with proteins containing the KDEL sequence. Detects three bands of ~94kD, ~78kD and ~55kD by Western Blot.\nSpecies Crossreactivity: mammals, birds, insects, plants and yeast