Glucose Regulated Protein 78 Receptor (Grp78 Receptor, BiP, KDEL)

KDEL receptor is a 26kD integral membrane protein that is responsible for the retrieval of soluble ER luminal proteins bearing the tetrapeptide, KDEL, from the Golgi apparatus (2,3). KDEL receptor exhibits extensive sequence identity to yeast protein Erd2p, which is a receptor for proteins containing the tetrapeptide HDEL, the yeast ER retention signal (2,3). KDEL receptor is localized to the cis Golgi and the intermediate compartments (3). KDEL receptor interacts with the GTPase-activating protein (GAP) of ARF1, a small GTP-binding protein involves in COPI-dependent vesicle transport (4). KDEL receptor may recruit cytosolic ARF1 to membranes and regulate vesicle budding (4).\n\nApplications:\nSuitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot (Colorimetric): 1:125\nImmunocytochemistry: 1:500\nOptimal dilution to be determined by researcher.\n\nPositive Controls:\nHeLa Heat Shocked Cell Lysate, H1838-01\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G3057-25

Size

25ul

Applications

ICC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, NHP, Prc, Rabbit, Sheep, Xen/Amph

Form

Suppied as a liquid in PBS, 0.09% azide, before the addition of glycerol to 40%.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. Tang, B.L., Low, S.H., Hauri, HP, Hong, W. (1994) Eur. J. Cell Biol. 65: 298-304. 2. Lewis, M.J. and Pelham, H.R. (1990) Nature 348: 162-163. 3. Tang, B.L., Wong,S.H, Qi, X.L., Low, S.H., Hong, W. (1993) J. Cell Biol. 120: 325-328. 4. Aoe, T., Cukierman, E., Lee, A., Cassel, D., Peters, P.J., Hsu, V.W. (1997) EMBO J. 16: 7305-7316. 5. Tang, B.L. (1994) Eur. J. Cell Biol. 65: 298-304.

Additional Info

Recognizes bovine KDEL at ~25kD. Species Crossreactivity: human, monkey, mouse, rat, hamster, rabbit, porcine, sheep, canine, chicken, Drosphila and Xenopus.