Glucose Regulated Protein 94 (Grp94, gp96)

Glucose-regulated protein 94, also known as Grp94 or gp96, is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which belongs to the Hsp90 family of molecular chaperones. Grp94 possesses the COOH-terminal tetrapeptide Lys-Asp-Glu-Leu (KDEL), a sorting signal thought to lead to the retention of proteins in the pre-Golgi compartments (1). Grp94 expression is upregulated by stress conditions which promote protein misfolding or unfolding (2). In addition to a homeostatic role in protein folding and assembly, Grp94 can function in the intracellular trafficking of peptides (3,4). Grp94 and Hsp90 share high sequence identity and presumably identical adenosine nucleotide-dependent modes of regulation. Hsp90 and Grp94 may differ in their nucleotide binding properties. The N-terminal domain of eukaryotic Hsp90 proteins contains a conserved adenosine nucleotide binding pocket which also serves as the binding site for the Hsp90 inhibitors geldanamycin and radicicol. . Recent data has identified a ligand dependent regulation of Grp94 function and suggest a model whereby Grp94 function is regulated through a ligand-dependent conversion of Grp94 from an inactive to an active conformation (5, 6).\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000\nImmunoprecipitation: 1:80\nOptimal dilutions to be determined by researcher.\n\nPositive Controls:\nMouse Brain Tissue Extract or Rat Brain Tissue Extract\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G3057-42

Size

50ug

Applications

ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Peter, F., Nguyen, Van P. ,Soling, H.D. (1992) J. Biol. Chem. 267: 10631-10637. 1. Peter, F., Nguyen, Van P. ,Soling, H.D. (1992) J. Biol. Chem. 267: 10631-10637. 2. Little, E., Ramakrishnan, M., Roy, B., Gazit, G., Lee, A.S. (1994) Crit. Rev. Eukaryot. Gene. Expr. 4: 1-18. 3. Nicchitta, C.V. (1998) Curr. Opin. Immunol. 10: 103-109. 4. Srivastava, P.K., Menoret, A., Basu, S., Binder, R.J., McQuade, K.L. (1998) Immunity 8: 657-665. 5. Wassenberg, J.J., Reed, R.C., Nicchitta, C.V. (2000) J. Biol. Chem. 6. Rosser, M F.N., Nicchitta, C.V. (2000) J. Biol. Chem. 7. Kobayashi, T., Ogawa, S., Yura, T., Yangi, H. (2000) Biochem. Biophys. Res. Comm. 267: 831-837. 8. Maidji, E., Tugizov, S., Abenes, G., Jones, T., Pereira, L. (1998) J. Virology 72: 5717-5727. 9. Genbank Accession Number NP_035761. 10. Chen, X., Ding, Y., Liu, C.G., Mikhail, S., Yang, C.S. (2002) Carcinogenesis 23(1): 123-130.

Additional Info

Recognizes a ~94kD protein, corresponding to the apparent molecular mass of Grp94 on SDS-PAGE immunoblots. Species Crossreactivity: human (weakly), mouse, rat, bovine. Antibody specificity is confirmed by peptide inhibition studies.