alpha-Glucosidase breaks down alpha-1,4 linked polysaccharides to glucose, which can be utilized as a source of energy. In the biotechnology industry, alpha-glucosidase is used to produce glucose from intermediate breakdown products of starch hydrolysis generated by enzymes such as amylase. Pompe disease, one of the 12 known glycogen storage diseases, is an autosomal recessive metabolic disorder attributed to alpha-glucosidase deficiency. In this disease, glycogen accumulates in the lysosomes, resulting in progressive muscle weakness, heart failure and other neurological symptoms.
Sample Type:
Serum, saliva, tissue and cell culture.
Intended Use:
Measurement of a-Glucosidase activity in biological samples and screening alpha-Glucosidase inhibitors.
Test Principle:
In the a-Glucosidase Activity Colorimetric BioAssay(TM) Kit, alpha-Glucosidase hydrolyzes the Substrate Mix to release the p-nitrophenol that can be measured colorimetrically (OD = 410nm). This is an easy, quick and high-throughput capable kit that can measure 0.1-10mU of alpha-glucosidase activity in a variety of samples.
Kit Components:
alpha-Glucosidase Assay Buffer, 1x25ml
alpha-Glucosidase Substrate Mix, 1x300ul
alpha-Glucosidase Positive Control, 1x vial
p-Nitrophenol Standard (100mM), 1x100ul
Storage and Stability:
Store powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Quick Search
Products 
