Glucosidase, apha Activity BioAssay(TM) Kit (Colorimetric)

Supplier:

United States Biological

Cat no: 167839

Glucosidase, apha Activity BioAssay(TM) Kit (Colorimetric)

alpha-Glucosidase breaks down alpha-1,4 linked polysaccharides to glucose, which can be utilized as a source of energy. In the biotechnology industry, alpha-glucosidase is used to produce glucose from intermediate breakdown products of starch hydrolysis generated by enzymes such as amylase. Pompe disease, one of the 12 known glycogen storage diseases, is an autosomal recessive metabolic disorder attributed to alpha-glucosidase deficiency. In this disease, glycogen accumulates in the lysosomes, resulting in progressive muscle weakness, heart failure and other neurological symptoms. \n\nSample Type:\nSerum, saliva, tissue and cell culture.\n\nIntended Use:\nMeasurement of a-Glucosidase activity in biological samples and screening alpha-Glucosidase inhibitors.\n\nTest Principle:\nIn the a-Glucosidase Activity Colorimetric BioAssay(TM) Kit, alpha-Glucosidase hydrolyzes the Substrate Mix to release the p-nitrophenol that can be measured colorimetrically (OD = 410nm). This is an easy, quick and high-throughput capable kit that can measure 0.1-10mU of alpha-glucosidase activity in a variety of samples.\n\nKit Components:\nalpha-Glucosidase Assay Buffer, 1x25ml\nalpha-Glucosidase Substrate Mix, 1x300ul\nalpha-Glucosidase Positive Control, 1x vial\np-Nitrophenol Standard (100mM), 1x100ul\n\nStorage and Stability:\nStore powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

167839

Size

100Tests

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

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