Glucosidase II, alpha

alpha-Glucosidase II is a 110kD glycosylated endoplasmic reticulum resident protein that is involved in trimming of glucose from newly synthesized glycosylated proteins in the endoplasmic reticulum (ER). Together with an 80kD beta subunit, these polypeptides remove two alpha-1,3-linked glucose from high mannose oligosaccharides linked to asparagine residue on glycoprotein. alpha-Glucosidase II mediated glucose trimming is necessary for the interaction of substrates such as influenza virus hemagglutinin (HA), MHC Class I , and T-cell antigen receptor (TCR) with calnexin, an ER resident chaperone that is specific for monoglucosylated glycoproteins.\n\nApplications:\nSuitable for use in Immunoprecipitation and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunoprecipitation: 1:25\nWestern Blot (Colorimetric): 1:250; Note: It is recommended for Western Blot applications, that the secondary antibody be quenched by adding normal sera (of an identical species to secondary host) to the diluent.\nOptimal dilutions to be determined by researcher.\n\nPositive Controls:\nRat Brain Tissue Extract\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G3059-50

Size

25ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Can, NHP, Prc, Rabbit, Sheep

Form

Supplied as a liquid, whole serum, before the addition of glycerol to 40%.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Strous, G.J., et al., J. Biol. Chem. 262: 3620-3625 (1987). 2. Trombetta, E.S., et al., J. Biol. Chem. 271: 27,509-27,516 (1996). 3. Hammond, C., et al., Proc. Natl. Acad. Sci. USA 91: 913-917 (1994). 4. Balow, J.P., et al., J. Biol. Chem. 270: 29,025-29,029 (1995). 5. Kearse, K.P., et al., EMBO J. 13: 3678-3686 (1994). 6. Baldwin, T.A., et al., J. Biol. Chem. 275(41): 32,071-32,076 (2000). 7. Baldwin, T.A. & Ostergaard, H.L., J. Biol. Chem. 277(52): 50,333-50,340 (2002).

Additional Info

Detects a ~110kD protein, corresponding to the apparent molecular mass of alpha-glucosidase II on SDS-PAGE. Species Crossreactivity: Human, mouse, rat, bovine, canine, hamster, monkey, porcine, rabbit and sheep.