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GLUL, aa1-373, Recombinant, Human (GLNS, Glutamate-ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59)

Cat no: G7121-04P

GLUL, aa1-373, Recombinant, Human (GLNS, Glutamate-ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59)

Glutamine synthetase (GLUL), which is therefore able to regulate intracellular concentrations of glutamate. GLUL catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling. GLUL is essential for proliferation of fetal skin fibroblasts and plays an important role in controlling body pH by removing ammonia from circulation. Mutations in GLUL are associated with congenital glutamine deficiency. \n\nSource:\nRecombinant corresponding to aa1-373 of human GLUL 6x his tagged, expressed in E.coli. \n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MTTSASSHLN KGIKQVYMSL PQGEKVQAMY IWIDGTGEGL RCKTRTLDSE PKCVEELPEW NFDGSSTLQS EGSNSDMYLV PAAMFRDPFR KDPNKLVLCE VFKYNRRPAE TNLRHTCKRI MDMVSNQHPW FGMEQEYTLM GTDGHPFGWP SNGFPGPQGP \nYYCGVGADRA YGRDIVEAHY RACLYAGVKI AGTNAEVMPA QWEFQIGPCE GISMGDHLWV ARFILHRVCE DFGVIATFDP KPIPGNWNGA GCHTNFSTKA MREENGLKYI EEAIEKLSKR HQYHIRAYDP KGGLDNARRL TGFHETSNIN DFSAGVANRS ASIRIPRTVG QEKKGYFEDR \nRPSANCDPFS VTEALIRTCL LNETGDEPFQ YKN\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G7121-04P

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 20% glycerol, 5mM DTT, 200mM sodium chloride.

Purity

~90% (SDS-PAGE)

References

1. Vermeulen T., et al, (2008) Arch Biochem Biophys. 478:96-102 2. Liaw SH., et al. (1995) Protein Sci. 4(11): 2358-65.

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