GluR1, phosphorylated (Ser831) (Glutamate Receptor)

GluR1 is a component of AMPA receptors (alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid receptors), which play a key role at excitatory synapses. GluR1 is widely expressed throughout the nervous system. Phosphorylation of Ser831 and Ser845 is reported to potentiate GluR1 function, suggesting a possible role in synaptic plasticity, learning and memory.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry (frozen). Other applications not tested. \n\nRecommended Dilution: \nWestern Blot: 1:1000, detecs a band of approximately 100 kD in rat brain lysates.\nOptimal dilution determined by the researcher. \n\nRecommended Secondary Reagents: \nSheep anti rabbit IgG:HRP\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G3500-04F

Size

150ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 0.1% BSA, 0.09% BSA and 50% glycerol.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Castellani, G.C. et al. (2001) A biophysical model of bidirectional synaptic plasticity: Dependence on AMPA and NMDA receptors. Proc. Natl. Acad. Sci. USA. 98: 12772 - 12777.

Additional Info

Specific for glutamate receptor subunit 1 (GluR1) when phosphorylated at serine 831. The specifity of this antibody has been shown by blocking studies in Western Blotting, whereby staining was blocked by the phospopeptide. No blocking was seen by the corresponding non-phosphopeptide. Species Cross Reactivity: Human, mouse and rat (100% identity with immunogen sequence).