GluR1, phosphorylated (Ser831) (Glutamate Receptor)

In the mammalian central nervous system, glutamate and its receptors (GluR) are the major excitatory neurotransmitter and receptor, respectively. Imbalances in glutamatergic function have been implicated in neuronal death following ischemia, hypoglycemia or anoxia, epilepsy, and in neurodegenerative disorders. Based on their activation by different pharmacologic agonists GluR have been classified into two major superfamilies: the ligand-gated ion channel receptors referred to as ionotropic GluR (iGluR), and the G-protein-coupled receptors referred to as metabotropic GluR (mGluR). The iGluR respond to selective agonists and are thus defined as the N-methyl-D-aspartate (NMDA) receptors, the alphaamino-3-hydroxy-5-methyl-isoxasole-4-propionate (AMPA) responsive receptors (GluR1, 2, 3 and 4) and the kainate responsive receptors (GluR5 and 6). GluR1 is phosphorylated on multiple sites, all are located on the C-terminal of the protein. Ligand induced phosphorylation\nof GluR1 seems to be site-specific. In one study, using transfected HEK cells and neurons in culture, cyclic AMP-dependent protein kinase specifically phosphorylates Ser845 of GluR11. In addition, protein kinase C specifically phosphorylates Ser831 of GluR12. The phosphorylation pattern of GluR1 by PKA and PKC is consistent with the study of AMPA-Rs in cortical neurins and GluR1 expressed in 293 cells. These results suggested that the function of GluR1 can be regulated by protein phosphorylation.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

G3500-06C

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM NaCl, 0.1mg/ml BSA, and 50% glycerol.

P Type

Pab

Purity

Purified by epitope affinity chromatography.

Additional Info

Recognizes the rat ~100kD GluR1 protein, phosphorylated at Ser831, and does not react with the non-phosphorylated form of the GluR protein. Reactivity has been confirmed in rat brain hippocampal homogenate.