GluR1, phosphorylated (Ser845) (Glutamate Receptor)

In the mammalian central nervous system, glutamate and its receptors (GluR) are the major excitatory neurotransmitter and receptor, respectively. Imbalances in glutamatergic function have been implicated in neuronal death following ischemia, hypoglycemia or anoxia, epilepsy, and in neurodegenerative disorders. Based on their activation by different pharmacologic agonists GluR have been classified into two major superfamilies: the ligand-gated ion channel receptors referred to as ionotropic GluR (iGluR), and the G-protein-coupled receptors referred to as metabotropic GluR (mGluR). The iGluR respond to selective agonists and are thus defined as the N-methyl-D-aspartate (NMDA) receptors, the alphaamino-3-hydroxy-5-methyl-isoxasole-4-propionate (AMPA) responsive receptors (GluR1, 2, 3 and 4) and the kainate responsive receptors (GluR5 and 6). GluR1 is phosphorylated on multiple sites, all are located on the C-terminal of the protein. Ligand induced phosphorylation of GluR1 seems to be site-specific. In one study, using transfected HEK cells and neurons in culture, cyclic AMP-dependent protein kinase specifically phosphorylates Ser845 of GluR11. In addition, protein kinase C specifically phosphorylates Ser831 of GluR12. The phosphorylation pattern of GluR1 by PKA and PKC is consistent with the study of AMPA-Rs in cortical neurins and GluR1 expressed in 293 cells. These results suggested that the function of GluR1 can be regulated by protein phosphorylation.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Dot Blot. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000\nDot Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: Rat brain hippocampal homogenate.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G3500-06D

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by epitope affinity chromatography.

Isotype

IgG

References

1. Roche KW, et al. Neuron 16(6):1179-88, 1996. 2. Barria A, et al. J Biol Chem 272(52):32727-30, 1997. 3. Chao SZ, et al. J Neurochem 81(5):984-92, 2002. 4. Banke TG, et al. J Neurosci 20(1):89-102, 2000. 5. Blackstone C, et al. J Neurosci 14(12):7585-93, 1994.

Additional Info

Recognizes the ~100kD rat GluR1 protein, phosphorylated at Ser845. Does not react with the non-phosphorylated form of the GluR protein.