GluR1, phosphorylated (Ser845) (Glutamate Receptor)

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct subunits GluR1-4 and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR1 subunit is widely expressed throughout the nervous system. GluR1 is also potentiated by phosphorylation at Ser845. In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory. \n\nApplications: \nApplications include dot blots and Western blots. \n\nRecommended Dilution: \nDot blot: 1:500-1:2000\nWestern blot: 1:500-1:2000\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

G3500-06E

Size

150ul

Applications

WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by sequential chromatography on a Protein A column and phospho- and non-phospho-peptide affinity columns.

Isotype

IgG

References

1. Lee, H.K., Takamiya, K., Han, J.S., Man, H.Y., Kim, C.H., Rumbaugh, G., Yu, S., Ding, L., He, C., Petralia, R.S., Wenthold, R.J., Gallagher, M. and Huganir, R.L., Cell 112 (2003) 631 - 643. \n2. Banke, T.G., Bowie, D., Lee, H.K., Huganir, R.L., Schousboe, A., and Traynelis, S.F. J. Neurosci. 20 (2000) 89 - 102. \n3. Barria, A., Muller, D., Derkach, V., Griffith, L.C. and Soderling, T.R., Science 276 (1997) 2042 - 2045. \n4. Roche, K.W., O'Brien, R.J., Mammen, A.L., Bernhardt, J., and Huganir, R.L., Neuron, 16 (1996) 1179 - 1188. \n5. McGlade-Mcculloh, E., Yamamoto, H., Tan, S.-E., Brickey, D.A. and Soderling, T.R. Nature (London) 362 (1993) 640 - 642.

Additional Info

Recognizes rat GluR1 phosphorytlated at Ser845 at 100kD. Species sequence homology: Human and mouse.