GluR2/3 (Glutamate receptor)

Glutamate receptor type 2 (GluR2) is one of the four subunits of alpha-amino-5-hydroxy-3-methyl-4-isoxazole propionic acid (AMPA) receptor. AMPA receptor is a non-NMDA-type ionotropic transmembrane receptor for glutamate that mediates fast synaptic transmission in the central nervous system (CNS (1). AMPA receptors are composed of four types of subunits, designated as GluR1, GluR2, GluR3, and GluR4 (alternatively called GluRA-D), which combine to form tetramers (2). The complex comprising of GluR2 and GluR3 is one of the two predominant forms in the adult hippocampus. All four GluRs interact with specific cytoplasmic protein through their C-terminal tails. Although in contrast to GluR1 and GluR4, GluR3 and GluR2 have a short C-terminal tail (3).\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunoprecipitation: 1:100\nImmunohistochemistry: 1:100-1:250\nImmunocytochemistry: 1:100-1:250\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G3500-15C

Size

100ul

Applications

ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Pellegrini DE, et al. Proc Natl Acad Sci 88:4157-4161 2001. 2. Boehm J, et al. Biochem Soc Trans 33:1334-1336. 2005. 3. Dingledine R, et al. Pharm Review 51:7-62. 1999.

Additional Info

Recognizes both GluR2/3 phosphorylated on Serine 880/891 of the mature human, and also unphosphorylated Glur-2/3. Species Crossreactivity: Mouse, rat.