GluR3 (Glutamate Receptor)

Glutamate is the major excitatory neurotransmitter in the mammalian central nervous system. Regulation of fast excitatory synaptic neurotransmission is mediated primarily by ionotropic glutamate receptors (iGluR); ligand-gated cation (principally Ca2+) channels found on postsynaptic neurons throughout the central nervous system. Pharmacologic properties and amino acid sequence similarities allow classification of iGluR into three main subfamilies named for their principal agonists: 1) NMDA (N-methyl-D-aspartate) receptors, and the two non-NMDA receptor subfamilies,\n2) AMPA (a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) receptors and 3) kainate. iGluR are oligomeric protein complexes comprised of 4 or 5 subunits, with the subunit composition determining specific electrophysiological properties. All NMDA receptors contain one NR1 subunit (encoded by a single gene producing 8 identified splice variants) and several NR2 subunits (four genes, A-D). AMPA and kainate receptors are comprised of subunits GluR1-4 (AMPA) and subunits GluR5-7, KA1 and KA2 (kainate).\n\nApplications:\nSuitable for use in Western Blot, Immunocytochemistry, Immunofluorescence and Electron Microscopy. Other applications have not been tested.\n\nRecommended Diluton:\nWestern Blot: 5ug/ml \nImmunocytochemistry: 1-3ug/ml \nImmunofluorescence: 1-3ug/ml\nImmunogold labeling/Electron Microscopy: 1-3ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

G3500-19

Size

100ug

Applications

FC, ICC, IF, WB

Hosts

Mouse

Reactivities

Hum, Rat, Moll

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A chromatography.

Isotype

IgG1

References

.Moga DE, et al. J Comp Neurol 462:15-28 (2003).

Additional Info

Recognizes rat Glutamate Receptor 3 (GluR3) at ~98kD. Does not react with cells transfected with GluR1, GluR2 or GluR4. Species Crossreactivity: human, monkey. Reactivity has been confirmed with HEK293 cells transfected with AMPA GluR cDNAs as well as human, rat, and macaque monkey brain extracts by Western Blot and rat hippocampus by immunofluorescence, immunocytochemistry, and immunogold labeling/electron microscopy.